Thursday, July 19, 2007


I finally did one run of a PCR entirely on my own - from taking the DNA, to putting in the master mix, to running the gel, and I obtained a result! Feels good to have known that I did the PCR properly - next step is for me to practice extracting DNA.

Next week, I've got a number of things I want to learn how to do, and one of them is to properly thaw and culture OP9 cells. (OP9 cells are stromal cells which have an "immortalization" mutation; they are grown as cells that help NK cells mature.)

This is what the gel looks like:

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